A novel nanosensing film composed of Gold, Copper, platinum nanoparticles and Aniline is fabricated using cyclic voltammetry for the detection of Neurotransmitters.. Neurotransmitter plays a vital role in human system and hence its determination is of thrust areas of research. In this regard, the high surface area to volume of the nanostructured particles with Aniline deposited on GC electrode is utilized for the for efficient electro catalytic oxidation of neurotransmitter molecules in the presence of interfering species. The nanocomposite film is utilized towards the oxidation of biochemical compounds like dopamine. The catalytic property of the nanomaterials modified electrodes is probed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Such nanomaterials modified electrodes can be used as a biosensor with high selectivity, sensitivity and detection of submicromolar concentrations of dopamine with practical applications to real samples.