ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES AGAINST RESISTANT AND NON RESISTANT CLINICAL ISOLATES OF DIFFERENT BACTERIA

SHOEB MUSTAFA*, HARIS M KHAN and INDU SHUKLA

DEPARTMENT OF MICROBIOLOGY, J.N.MEDICAL COLLEGE AND HOSPITAL, A.M.U, ALIGARH, INDIA

E-MAIL: shoebmustafa82@indiatimes.com

Nanotechnology is expected to open new avenues to fight and prevent disease using atomic scale tailoring of materials. Among the most promising nanomaterials with antibacterial properties are metallic nanoparticles, which exhibit increased chemical activity due to their large surface to volume ratios and crystallographic surface structure. The study of bactericidal nanomaterials is particularly timely considering the recent increase of new resistant strains of bacteria to the most potent antibiotics. This has promoted research in the well known activity of silver ions and silver-based compounds, including silver nanoparticles. The present work studies the antibacterial behavior of silver nanoparticles against different bacterial isolates identified in our department showing either no or variable degree of resistance to different antibiotics with special emphasis on ESBL (Extended Spectrum Beta Lactamase) producing strains of Escherichia coli and Klebsiella spp. We assessed the activity of silver nanoparticles against different bacteria using turbidimetric analysis (Spectrophotometric analysis), broth dilution method and diffusion method with positive results. Organisms from clinical isolates with both the resistant and non-resistant strains were tested by us, namely Escherichia coli, Klebsiella spp., Proteus mirabilis, Proteus vulgaris, Pseudomonas spp., Salmonella typhi, Shigella dysenteriae, Shigella flexneri, Staphyloccus aureus, Streptococcus viridans, Vibrio cholerae and Oxacillin Resistant Staphyloccus aureus. Atomic Force Microscopy (AFM) was done to assess changes in morphology of bacteria. AFM analyses of the bacteria after treatment with Silver nanoparticles showed damage to the cell wall of the bacteria.

Keywords: Silver nanoparticles; Resistant bacteria; Extended Spectrum Beta Lactamase; Spectrophotometric analysis; Broth dilution method; Diffusion method; Atomic Force Microscopy.

Corresponding author contact

Dr SHOEB MUSTAFA, DEPARTMENT OF MICROBIOLOGY, J.N.MEDICAL COLLEGE AND HOSPITAL, A.M.U, ALIGARH, U.P, 202001, INDIA

Phone No: 91-9219880360

E-MAIL: shoebmustafa82@indiatimes.com